P. Panailidou, L. Lotos, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece; A. Olmos, A.B. Ruiz-Garcia, F. Moran, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain; C.G. Orfanidou, C-L. Sassalou, N.I. Katis and V.I. Maliogka, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece 

Santiago, Chile, April 9-12, 2018

It is a great pleasure for me to invite you to the 19th Conference of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), to be held in Santiago of Chile, on 9-12 of April, 2018. Its venue will be Santa Carolina Winery warehouse. We feel honored to host this meeting for the first time in South America, and hope that you will enjoy your staying in our beautiful city, and make of this meeting an excellent opportunity for presenting your research and establish networks. 

Introduction: Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., FabavirusLuteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed

Abstract: Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., FabavirusLuteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed

Abstract: Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches.

Felix Moran; Antonio Olmos; Leonidas Lotos; Lukas Predajňa; Nikolaos Katis; Miroslav Glasa; Varvara Maliogka; Ana B. Ruiz-Garcia 

First Report of Grapevine Latent Viroid Infecting Grapevine (Vitis vinifera L.) 1 in Italy
S. Rotunno, A.M. Vaira, D. Marian, A. Schneider and S. Raimondi , Institute for Sustainable Plant Protection, Strada delle Cacce 73, 10135, Torino, Italy; F. Di Serio and B. Navarro, Institute for Sustainable Plant Protection, Bari Unit, via Amendola, 122/D, 70126, Bari, Italy; L. Miozzi*, Institute for Sustainable Plant Protection, Strada delle Cacce 73, 10135, Torino, 

Igor Koloniuk; Tatiana Sarkisova; Karel Petrzik; Ondrej Lenz; Jaroslava Pribylová; Jana Fránová; Josef Špak; Leonidas Lotos; Christina Beta; Asimina Katsiani; Thierry Candresse; Varvara I. Maliogka

During their lifetime, perennial woody plants are expected to face multiple infection events. Furthermore, multiple genotypes of individual virus species may co-infect the same host. This may eventually lead to a situation where plants harbor complex communities of viral species/strains. Using high-throughput sequencing, we describe co-infection of sweet and sour cherry trees with diverse genomic variants of two closely related viruses, namely prunus virus F (PrVF) and cherry virus F (CVF).

Mar 08, 2018

Plant Disease

Written by

First report of avocado sunblotch viroid (ASBVd) naturally infecting avocado (Persea americana) in Greece

Mr. Leonidas Lotos
Aristotle University of Thessaloniki, AgriculturePlant Pathology LaboratoryPOB 269University Campus, Thessaloniki, Greece54124+302310998855; This email address is being protected from spambots. You need JavaScript enabled to view it.

Dr. Nektarios Kavroulakis
Institute for Olive Tree and Subtropical Plants of Chania, Laboratory of Subtropical PlantsAgrokipioChania, Chania, Greece7100; This email address is being protected from spambots. You need JavaScript enabled to view it.

 

VirFree (H2020-MSCA-RISE-2016-Virus free fruit nurseries) © All Rights Reserved | This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 734736.