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The Technical workshop entitled "Nanobody workshop BIOREBA AG and CNRS", has been performed successfully on 27 and 28 March 2019 at BIOREBA AG facilities, Reinach, Switzerland 

P. Panailidou, L. Lotos, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece; A. Olmos, A.B. Ruiz-Garcia, F. Moran, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain; C.G. Orfanidou, C-L. Sassalou, N.I. Katis and V.I. Maliogka, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece 

Santiago, Chile, April 9-12, 2018

It is a great pleasure for me to invite you to the 19th Conference of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), to be held in Santiago of Chile, on 9-12 of April, 2018. Its venue will be Santa Carolina Winery warehouse. We feel honored to host this meeting for the first time in South America, and hope that you will enjoy your staying in our beautiful city, and make of this meeting an excellent opportunity for presenting your research and establish networks. 

The Technical workshop entitled "Plant virus diagnostic application of RNA high throughput sequencing to phytosanitary certification procedures", has been performed successfully on7,8 and 9 November 2018 at IPSP-CNR, Bari, ITALY

 

Introduction: Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., FabavirusLuteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed

""Technical workshop on Plant virus diagnostic application of RNA high throughput sequencing to phytosanitary certification procedures. 7-9 November 2018, IPSP-CNR, Bari, ITALY" 

Abstract: Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., FabavirusLuteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed

Abstract: Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches.

Project

Jul 11, 2018
 

Introduction

 

Fruit trees and grapevine are propagated vegetatively and are often grafted
As a result they suffer from a high number of pathogens such as viruses and viroids, with some of them causing severe yield losses and reducing the productive life of the affected plants
These pathogens cannot be controlled by the application of chemicals therefore the most efficient way to combat them is the production and commercialization of high quality pathogen-tested propagative material
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Objectives of VirFree

 

VirFree brings together participants from both academia and privatecompanies to collaborate through their expertise on the following objectives:

     

to identify new viral and viroid strains or species affecting fruit trees and grapevine

to optimize existing and develop novel detection methods

to improve propagation and sanitation methods for producing high quality (virus-tested) plant material of fruit trees and grapevine

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 Approach

 

Diagnostic tools currently used in certification schemes will becombined with cutting edge technologies such as NGS and Nanobodies
 
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 Expected benefits

 

 Training of a new generation of researchers to fulfill the needs of both industrial and academic sectors

Enrich the knowledge on viruses/viroids associated with different diseases

Improve the sensitivity of detection methods

Development of new detection products

Improvement of the disseminated propagative material

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""The VirFree Midterm meeting has been performed at the School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece on 4 and 5 June 2018." 

 

 

VirFree brings together participants from both academia and privatecompanies to collaborate through their expertise on the following objectives

rfree, NGS, Nanobodies, Fruit tree viruses, viroids, virus elimination ,http://www.virfree.eu ,virfree,virfree.eu , This email address is being protected from spambots. You need JavaScript enabled to view it.

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VirFree (H2020-MSCA-RISE-2016-Virus free fruit nurseries) © All Rights Reserved | This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 734736.