Grapevine (Vitis vinifera) is one of the most ancient crops cultivated worldwide for the production of both fresh grapes and wine, which represents an important source of income for many countries. Europe is the leading global wine producer, with about 50% of the world’s vine-growing area, with 3.3 million ha (Food and Agriculture Organization of the United Nations Statistics Division, FAOSTAT, 2018). Grapevine can be affected by virus and virus-like diseases that may significantly reduce the productive life of the plants as well as the grape production in terms of the quantity and quality of the berries . In fact, the grapevine has been described as the crop that hosts the largest number of plant viruses, with more than 85 viral species known to infect this important crop to date [2,3].
The number of viruses identified in sweet cherry has been constantly increasing over the last few years, following the broad application of high-throughput sequencing (HTS). Some of these were reported to cause leaf symptoms and yield reduction.
Grapevine Roditis leaf discoloration-associated virus (GRLDaV) is an emerging grapevine pathogen included in the European and Mediterranean Plant Protection Organization (EPPO) alert list due to its ability to damage grapevine crops and cause production losses. This work aimed to develop a specific and reliable diagnostic tool that would contribute to preventing the spread of this pathogen. Therefore, a TaqMan real-time quantitative PCR was developed. The method was validated according to EPPO guidelines showing a high degree of analytical sensitivity, analytical specificity, selectivity, and repeatability and reproducibility.
The sensitivity of this method is much higher than the sensitivity reached by previously reported methods even when tested in crude extracts, which could allow rapid testing by avoiding nucleic acid extraction steps. The method was also able to detect GRLDaV isolates from all the geographic origins reported so far, despite their high degree of genetic diversity. In addition, this new technique has been successfully applied for the quantitative detection of GRLDaV in plant material and two mealybug species, Planococcus citri and Pseudococcus viburni. In conclusion, the methodology developed herein represents a significant contribution to the diagnosis and control of this emerging pathogen in grapevine.
The number of viruses identified in sweet cherry has been constantly increasing over the last few years, following the broad application of high throughput sequencing (HTS). Some of these were reported to cause leaf symptoms and yield reduction. In 2009-2013, surveys were performed on sweet cherry orchards located in Northern Greece for the presence of Betaflexiviridae viruses using generic and specific molecular assays (Foissac et al., 2005).
The Technical workshop entitled "Nanobody workshop BIOREBA AG and CNRS", has been performed successfully on 27 and 28 March 2019 at BIOREBA AG facilities, Reinach, Switzerland
P. Panailidou, L. Lotos, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece; A. Olmos, A.B. Ruiz-Garcia, F. Moran, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain; C.G. Orfanidou, C-L. Sassalou, N.I. Katis and V.I. Maliogka, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
Santiago, Chile, April 9-12, 2
It is a great pleasure for me to invite you to the 19th Conference of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), to be held in Santiago of Chile, on 9-12 of April, 2018. Its venue will be Santa Carolina Winery warehouse. We feel honored to host this meeting for the first time in South America, and hope that you will enjoy your staying in our beautiful city, and make of this meeting an excellent opportunity for presenting your research and establish networks.
The Technical workshop entitled "Plant virus diagnostic application of RNA high throughput sequencing to phytosanitary certification procedures", has been performed successfully on7,8 and 9 November 2018 at IPSP-CNR, Bari, ITALY
VirFree (H2020-MSCA-RISE-2016-Virus free fruit nurseries) © All Rights Reserved | This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 734736.