Grapevine Roditis leaf discoloration-associated virus (GRLDaV) is an emerging grapevine pathogen included in the European and Mediterranean Plant Protection Organization (EPPO) alert list due to its ability to damage grapevine crops and cause production losses. This work aimed to develop a specific and reliable diagnostic tool that would contribute to preventing the spread of this pathogen. Therefore, a TaqMan real-time quantitative PCR was developed. The method was validated according to EPPO guidelines showing a high degree of analytical sensitivity, analytical specificity, selectivity, and repeatability and reproducibility.

The sensitivity of this method is much higher than the sensitivity reached by previously reported methods even when tested in crude extracts, which could allow rapid testing by avoiding nucleic acid extraction steps. The method was also able to detect GRLDaV isolates from all the geographic origins reported so far, despite their high degree of genetic diversity. In addition, this new technique has been successfully applied for the quantitative detection of GRLDaV in plant material and two mealybug species, Planococcus citri and Pseudococcus viburni. In conclusion, the methodology developed herein represents a significant contribution to the diagnosis and control of this emerging pathogen in grapevine.

The number of viruses identified in sweet cherry has been constantly increasing over the last few years, following the broad application of high throughput sequencing (HTS). Some of these were reported to cause leaf symptoms and yield reduction. In 2009-2013, surveys were performed on sweet cherry orchards located in Northern Greece for the presence of Betaflexiviridae viruses using generic and specific molecular assays (Foissac et al., 2005).

P. Panailidou, L. Lotos, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece; A. Olmos, A.B. Ruiz-Garcia, F. Moran, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain; C.G. Orfanidou, C-L. Sassalou, N.I. Katis and V.I. Maliogka, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece 

Santiago, Chile, April 9-12, 2018

It is a great pleasure for me to invite you to the 19th Conference of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), to be held in Santiago of Chile, on 9-12 of April, 2018. Its venue will be Santa Carolina Winery warehouse. We feel honored to host this meeting for the first time in South America, and hope that you will enjoy your staying in our beautiful city, and make of this meeting an excellent opportunity for presenting your research and establish networks. 

Introduction: Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., FabavirusLuteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed

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VirFree (H2020-MSCA-RISE-2016-Virus free fruit nurseries) © All Rights Reserved | This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 734736.